The smart Trick of columns used in HPLC analysis That No One is Discussing
C18 reveals the typical attributes of the alkyl group - non-polar and ionically inert in most popular disorders. Retention from a C18 stationary phase comes from weak van der Waals intermolecular power that attracts hydrophobic compounds. C18 along with other alkyl phases for instance C8 and C4 represent by far the most elementary style of reverse